Rat E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®

Cadherin-1, also known as CAM 120/80 or epithelial cadherin (E-cadherin) or uvomorulin, is a protein that in humans is encoded by the CDH1 gene. E-cadherin is a Ca (2+) -dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. It plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 284 297 279 284 7.56 2.6% Sample 2 1708 1726 1809 1726 1742 39.23 2.2% Sample 3 5563 5211 5926 5398 5524 263.13 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 284 297 279 284 7.56 2.6% Sample 1 278 284 297 279 284 7.56 2.6% Sample 2 1708 1726 1809 1726 1742 39.23 2.2% Sample 2 1708 1726 1809 1726 1742 39.23 2.2% Sample 3 5563 5211 5926 5398 5524 263.13 4.7% Sample 3 5563 5211 5926 5398 5524 263.13 4.7%

Cadherins, Calcium Binding Proteins, Calcium Signaling, Cancer, Cell Adhesion, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1708 5563 288 1866 5045 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1708 5563 288 1866 5045 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1708 5563 288 1866 5045 Mean (pg/ml) 278 1708 5563 288 1866 5045 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 Standard deviation 21.4 122.97 379.5%8 27.36 169.8 363.24 CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2% CV (%) 7.7% 7.2% 6.8% 9.5% 9.1% 7.2%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell junction. Cell membrane; Single-pass type I membrane protein. Endosome . Golgi apparatus, trans-Golgi network . Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane (By similarity) . .

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007 O.D. 0.055 0.106 0.161 0.258 0.448 0.797 1.365 2.007

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.