Human Relaxin 2 ELISA Kit PicoKine®

Relaxins are known endocrine and autocrine/paracrine hormones, belonging to the insulin gene superfamily. In humans there are three non-allelic relaxin genes, RLN1, RLN2 and RLN3, where RLN1 and RLN2 share high sequence homology. The active form of the protein encoded by this gene consists of an A chain and a B chain linked by disulfide bonds. Relaxin is produced by the ovary, and targets the mammalian reproductive system to ripen the cervix, elongate the pubic symphysis and inhibit uterine contraction. It may have additional roles in enhancing sperm motility, regulating blood pressure, controlling heart rate and releasing oxytocin and vasopressin. Relaxin 2 (RLN2) is a polypeptide hormone structurally related to insulin and insulin-like growth factors (IGFs) . However it does not interact with insulin receptors and has decidedly different biological properties. Relaxin 2 activates LGR 7 and LGR 8 relaxin receptors, that belong to the leucine-rich repeat-containing (LRR), G protein-coupled receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 17 19 24 1.86 7.8% Sample 2 107 77 103 114 100 4.21 4.2% Sample 3 422 439 442 489 448 11.92 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 29 17 19 24 1.86 7.8% Sample 1 31 29 17 19 24 1.86 7.8% Sample 2 107 77 103 114 100 4.21 4.2% Sample 2 107 77 103 114 100 4.21 4.2% Sample 3 422 439 442 489 448 11.92 2.7% Sample 3 422 439 442 489 448 11.92 2.7%

Isoform 1 is expressed in the ovary during pregnancy. Also expressed in placenta, decidua and prostate. Isoform 2 is relatively abundant in placenta. It is in much lower abundance in the prostate gland. Not detected in the ovary. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 107 422 19 95 323 Standard deviation 1.36 6.28 10.55 0.88 2.87 10.37 CV (%) 4.4% 5.9% 2.5% 4.6% 3.0% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 107 422 19 95 323 Standard deviation 1.36 6.28 10.55 0.88 2.87 10.37 CV (%) 4.4% 5.9% 2.5% 4.6% 3.0% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 107 422 19 95 323 Mean (pg/ml) 31 107 422 19 95 323 Standard deviation 1.36 6.28 10.55 0.88 2.87 10.37 Standard deviation 1.36 6.28 10.55 0.88 2.87 10.37 CV (%) 4.4% 5.9% 2.5% 4.6% 3.0% 3.2% CV (%) 4.4% 5.9% 2.5% 4.6% 3.0% 3.2%

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.057 0.105 0.208 0.41 0.819 1.513 2.338 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.057 0.105 0.208 0.41 0.819 1.513 2.338 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.012 0.057 0.105 0.208 0.41 0.819 1.513 2.338 O.D. 0.012 0.057 0.105 0.208 0.41 0.819 1.513 2.338

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.