Human AFP/Alpha-fetoprotein ELISA Kit PicoKine®

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25) . AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 105 109 107 104 5.38 5.1% Sample 2 643 732 682 710 691 33.25 4.8% Sample 3 2190 2290 2420 2404 2326 93.15 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 95 105 109 107 104 5.38 5.1% Sample 1 95 105 109 107 104 5.38 5.1% Sample 2 643 732 682 710 691 33.25 4.8% Sample 2 643 732 682 710 691 33.25 4.8% Sample 3 2190 2290 2420 2404 2326 93.15 4% Sample 3 2190 2290 2420 2404 2326 93.15 4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 643 2190 98 654 1986 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 98 643 2190 98 654 1986 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 98 643 2190 98 654 1986 Mean (pg/ml) 98 643 2190 98 654 1986 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 Standard deviation 4.56 47.58 100.74 5.58 56.24 129.09 CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5% CV (%) 4.8% 7.4% 4.6% 5.7% 8.6% 6.5%

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925 O.D. 0.003 0.137 0.248 0.486 0.777 1.260 1.704 1.925

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.