Mouse CCL19/MIP-3 beta ELISA Kit PicoKine®

Mouse CCL19/MIP-3 beta ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Ccl19 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta) . Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 32 28 32 30 1.65 5.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 3 384 401 402 407 398 8.67 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 32 28 32 30 1.65 5.5% Sample 1 30 32 28 32 30 1.65 5.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 2 161 166 173 167 166 4.26 2.5% Sample 3 384 401 402 407 398 8.67 2.1% Sample 3 384 401 402 407 398 8.67 2.1%

Synonyms

C-C motif chemokine 19; Epstein-Barr virus-induced molecule 1 ligand chemokine; EBI1 ligand chemokine; ELC; Small-inducible cytokine A19; Ccl19; Elc, Scya19

Gene Name

Ccl19

Gene ID

24047

UniProt

O70460

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G26-S108

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.

Applications

ELISA

Field of Research

Chemokines, G Protein Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 161 384 27 175 349 Mean (pg/ml) 30 161 384 27 175 349 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 Standard deviation 1.2 8.53 29.56 1.45 9.27 33.5 CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6% CV (%) 4% 5.3% 7.7% 5.4% 5.3% 9.6%

Sensitivity

<10 pg/mL

Function

Strongly chemotactic for naive (L-selectinhi) CD4 T- cells and for CD8 T-cells and weakly attractive for resting B- cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

11911 MW

Specificity

Natural and recombinant mouse CCL19

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-C motif chemokine 19

Subcellular Location

Secreted.

Protein Name

C-C motif chemokine 19

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909 O.D. 0.005 0.051 0.109 0.198 0.418 0.787 1.297 1.909

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.