Mouse CD23/FCER2 ELISA Kit PicoKine®

CD23, also known as Fc epsilon RII, or FcεRII, is the low-affinity receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 256 296 293 254 274 19.79 7.2% Sample 2 1831 1672 1609 1822 1733 95.68 5.5% Sample 3 5853 5387 5221 5268 5432 250.34 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 256 296 293 254 274 19.79 7.2% Sample 1 256 296 293 254 274 19.79 7.2% Sample 2 1831 1672 1609 1822 1733 95.68 5.5% Sample 2 1831 1672 1609 1822 1733 95.68 5.5% Sample 3 5853 5387 5221 5268 5432 250.34 4.6% Sample 3 5853 5387 5221 5268 5432 250.34 4.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 256 1831 5853 234 1881 5493 Standard deviation 11 137.32 345.32 12.87 156.12 373.52 CV (%) 4.3% 7.5% 5.9% 5.5% 8.3% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 256 1831 5853 234 1881 5493 Standard deviation 11 137.32 345.32 12.87 156.12 373.52 CV (%) 4.3% 7.5% 5.9% 5.5% 8.3% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 256 1831 5853 234 1881 5493 Mean (pg/ml) 256 1831 5853 234 1881 5493 Standard deviation 11 137.32 345.32 12.87 156.12 373.52 Standard deviation 11 137.32 345.32 12.87 156.12 373.52 CV (%) 4.3% 7.5% 5.9% 5.5% 8.3% 6.8% CV (%) 4.3% 7.5% 5.9% 5.5% 8.3% 6.8%

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.121 0.179 0.28 0.484 0.867 1.411 1.892 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.121 0.179 0.28 0.484 0.867 1.411 1.892 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.068 0.121 0.179 0.28 0.484 0.867 1.411 1.892 O.D. 0.068 0.121 0.179 0.28 0.484 0.867 1.411 1.892

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.