Mouse BMP-4 ELISA Kit PicoKine®

Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 54 54 58 54 1.92 3.5% Sample 2 250 270 229 249 249 14.5 5.8% Sample 3 897 924 940 993 938 35.01 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 54 54 58 54 1.92 3.5% Sample 1 53 54 54 58 54 1.92 3.5% Sample 2 250 270 229 249 249 14.5 5.8% Sample 2 250 270 229 249 249 14.5 5.8% Sample 3 897 924 940 993 938 35.01 3.7% Sample 3 897 924 940 993 938 35.01 3.7%

Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Nervous System Development, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 250 897 54 268 915 Standard deviation 2.65 17.5 52.92 3.07 19.83 53.98 CV (%) 5% 7% 5.9% 5.7% 7.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 250 897 54 268 915 Standard deviation 2.65 17.5 52.92 3.07 19.83 53.98 CV (%) 5% 7% 5.9% 5.7% 7.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 250 897 54 268 915 Mean (pg/ml) 53 250 897 54 268 915 Standard deviation 2.65 17.5 52.92 3.07 19.83 53.98 Standard deviation 2.65 17.5 52.92 3.07 19.83 53.98 CV (%) 5% 7% 5.9% 5.7% 7.4% 5.9% CV (%) 5% 7% 5.9% 5.7% 7.4% 5.9%

Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.025 0.048 0.1 0.195 0.408 0.834 1.787 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.025 0.048 0.1 0.195 0.408 0.834 1.787 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.002 0.025 0.048 0.1 0.195 0.408 0.834 1.787 O.D. 0.002 0.025 0.048 0.1 0.195 0.408 0.834 1.787

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.