Human CD47 ELISA Kit PicoKine®

Human CD47 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CD47 in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD47 (Cluster of Differentiation 47), also known as integrin associated protein (IAP), is a transmembrane protein that in humans is encoded by the CD47 gene. It belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα) . CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. Also CD47 is ubiquitously expressed in human cells and has found to be overexpressed in many different tumor cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 162 183 189 181 11.94 6.5% Sample 2 888 960 1024 1007 969 52.69 5.4% Sample 3 3988 4066 3941 3907 3975 59.64 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 193 162 183 189 181 11.94 6.5% Sample 1 193 162 183 189 181 11.94 6.5% Sample 2 888 960 1024 1007 969 52.69 5.4% Sample 2 888 960 1024 1007 969 52.69 5.4% Sample 3 3988 4066 3941 3907 3975 59.64 1.5% Sample 3 3988 4066 3941 3907 3975 59.64 1.5%

Synonyms

Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47; CD47; MER6

Gene Name

CD47

Gene ID

961

UniProt

Q08722

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-P139

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Applications

ELISA

Field of Research

Cancer, Cell Type Markers, Immunology, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 888 3988 208 813 3951 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 193 888 3988 208 813 3951 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 193 888 3988 208 813 3951 Mean (pg/ml) 193 888 3988 208 813 3951 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 Standard deviation 7.72 54.16 183.44 11.02 60.97 256.81 CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5% CV (%) 4% 6.1% 4.6% 5.3% 7.5% 6.5%

Sensitivity

<10 pg/mL

Function

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity) . Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

35214 MW

Specificity

Natural and recombinant human CD47

Gene Name Synonym

Leukocyte surface antigen CD47

Subcellular Location

Cell membrane ; Multi-pass membrane protein .

Sequence Similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Protein Name

Leukocyte surface antigen CD47

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905 O.D. 0.033 0.073 0.125 0.213 0.376 0.658 1.12 1.905

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.