Mouse VEGF-B ELISA Kit PicoKine®

Mouse VEGF-B ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Vegfb in cell culture supernatants, serum and plasma (heparin) . . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 68 79 79 74 5.04 6.8% Sample 2 247 261 242 260 252 8.2 3.2% Sample 3 978 875 882 968 925 47.44 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 68 79 79 74 5.04 6.8% Sample 1 70 68 79 79 74 5.04 6.8% Sample 2 247 261 242 260 252 8.2 3.2% Sample 2 247 261 242 260 252 8.2 3.2% Sample 3 978 875 882 968 925 47.44 5.1% Sample 3 978 875 882 968 925 47.44 5.1%

Synonyms

Vascular endothelial growth factor B; VEGF-B; VEGF-related factor; VRF; Vegfb; Vrf

Gene Name

Vegfb

Gene ID

22340

UniProt

P49766

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q20-A207 & Q20-R148

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Abundantly expressed in heart, brain, kidney and skeletal muscle.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 247 978 69 246 1037 Standard deviation 4.13 20.17 48.90 4.83 20.17 59.10 CV (%) 5.0% 7.5% 5.0% 7.0% 8.2% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 247 978 69 246 1037 Standard deviation 4.13 20.17 48.90 4.83 20.17 59.10 CV (%) 5.0% 7.5% 5.0% 7.0% 8.2% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 70 247 978 69 246 1037 Mean (pg/ml) 70 247 978 69 246 1037 Standard deviation 4.13 20.17 48.90 4.83 20.17 59.10 Standard deviation 4.13 20.17 48.90 4.83 20.17 59.10 CV (%) 5.0% 7.5% 5.0% 7.0% 8.2% 5.7% CV (%) 5.0% 7.5% 5.0% 7.0% 8.2% 5.7%

Sensitivity

<2 pg/mL

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

21914 MW

Specificity

Natural and recombinant mouse VEGF-B

Anticoagulant

Heparin

Gene Name Synonym

Vascular endothelial growth factor B

Subcellular Location

Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Sequence Similarities

Belongs to the PDGF/VEGF growth factor family.

Protein Name

Vascular endothelial growth factor B

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.051 0.088 0.177 0.357 0.767 1.626 2.999 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.051 0.088 0.177 0.357 0.767 1.626 2.999 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.017 0.051 0.088 0.177 0.357 0.767 1.626 2.999 O.D. 0.017 0.051 0.088 0.177 0.357 0.767 1.626 2.999

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.