Rat MMP-8/Neutrophil collagenase ELISA Kit PicoKine®

Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 238 216 228 210 223 10.81 4.8% Sample 2 1865 1657 1883 1979 1846 117.4 6.3% Sample 3 3841 4372 4478 4486 4294 265.52 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 238 216 228 210 223 10.81 4.8% Sample 1 238 216 228 210 223 10.81 4.8% Sample 2 1865 1657 1883 1979 1846 117.4 6.3% Sample 2 1865 1657 1883 1979 1846 117.4 6.3% Sample 3 3841 4372 4478 4486 4294 265.52 6.1% Sample 3 3841 4372 4478 4486 4294 265.52 6.1%

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 238 1865 3841 261 1840 3780 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 238 1865 3841 261 1840 3780 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 238 1865 3841 261 1840 3780 Mean (pg/ml) 238 1865 3841 261 1840 3780 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 Standard deviation 18.08 91.38 261.18 21.92 99.36 325.08 CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6% CV (%) 7.6% 4.9% 6.8% 8.4% 5.4% 8.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05 O.D. 0.011 0.074 0.145 0.297 0.57 1.038 1.58 2.05

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.