Human SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®

Sex hormone-binding globulin (SHBG) or sex steroid-binding globulin (SSBG) is a glycoprotein that binds to the two sex hormones: androgen and estrogen. The gene for SHBG is located on chromosome 17 on the short arm between the bands 17p12→p13. And this gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (nmol/L) Lot 2 (nmol/L) Lot 3 (nmol/L) Lot 4 (nmol/L) Mean (nmol/L) Standard Deviation CV (%) Sample 1 60 62 64 64 62 1.65 2.6% Sample 2 301 260 295 294 287 16.1 5.6% Sample 3 945 910 861 916 908 30.19 3.3% Lots Lot 1 (nmol/L) Lot 2 (nmol/L) Lot 3 (nmol/L) Lot 4 (nmol/L) Mean (nmol/L) Standard Deviation CV (%) Lots Lot 1 (nmol/L) Lot 2 (nmol/L) Lot 3 (nmol/L) Lot 4 (nmol/L) Mean (nmol/L) Standard Deviation CV (%) Sample 1 60 62 64 64 62 1.65 2.6% Sample 1 60 62 64 64 62 1.65 2.6% Sample 2 301 260 295 294 287 16.1 5.6% Sample 2 301 260 295 294 287 16.1 5.6% Sample 3 945 910 861 916 908 30.19 3.3% Sample 3 945 910 861 916 908 30.19 3.3%

Sex hormone-binding globulin; SHBG; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin; SHBG

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (nmol/L) 60 301 945 61 312 954 Standard deviation 3 18.66 60.48 3.66 22.46 67.73 CV (%) 5% 6.2% 6.4% 6% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (nmol/L) 60 301 945 61 312 954 Standard deviation 3 18.66 60.48 3.66 22.46 67.73 CV (%) 5% 6.2% 6.4% 6% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (nmol/L) 60 301 945 61 312 954 Mean (nmol/L) 60 301 945 61 312 954 Standard deviation 3 18.66 60.48 3.66 22.46 67.73 Standard deviation 3 18.66 60.48 3.66 22.46 67.73 CV (%) 5% 6.2% 6.4% 6% 7.2% 7.1% CV (%) 5% 6.2% 6.4% 6% 7.2% 7.1%

Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (nmol/ml) 0 0.031 0.062 0.125 0.25 0.5 1 2 O.D. 0.046 0.123 0.192 0.273 0.531 0.953 1.551 2.25 Concentration (nmol/ml) 0 0.031 0.062 0.125 0.25 0.5 1 2 O.D. 0.046 0.123 0.192 0.273 0.531 0.953 1.551 2.25 Concentration (nmol/ml) 0 0.031 0.062 0.125 0.25 0.5 1 2 Concentration (nmol/ml) 0 0.031 0.062 0.125 0.25 0.5 1 2 O.D. 0.046 0.123 0.192 0.273 0.531 0.953 1.551 2.25 O.D. 0.046 0.123 0.192 0.273 0.531 0.953 1.551 2.25

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.