Human LBP ELISA Kit PicoKine®

Human LBP ELISA Kit PicoKine® (96 Tests) . Quantitate Human LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1485 1561 1630 1640 1579 62.21 3.9% Sample 2 4533 5189 4438 4703 4715 289.25 6.1% Sample 3 23562 22985 21907 22035 22622 683.95 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1485 1561 1630 1640 1579 62.21 3.9% Sample 1 1485 1561 1630 1640 1579 62.21 3.9% Sample 2 4533 5189 4438 4703 4715 289.25 6.1% Sample 2 4533 5189 4438 4703 4715 289.25 6.1% Sample 3 23562 22985 21907 22035 22622 683.95 3% Sample 3 23562 22985 21907 22035 22622 683.95 3%

Synonyms

Lipopolysaccharide-binding protein; LBP; LBP

Gene Name

LBP

Gene ID

3929

UniProt

P18428

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-V481

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Detected in blood serum (at protein level) . .

Applications

ELISA

Field of Research

Defensin, Human Protein, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, TLR Signaling

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1485 4533 23562 1518 4575 21374 Standard deviation 66.82 271.98 1507.96 85 297.37 1432.05 CV (%) 4.5% 6% 6.4% 5.6% 6.5% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1485 4533 23562 1518 4575 21374 Standard deviation 66.82 271.98 1507.96 85 297.37 1432.05 CV (%) 4.5% 6% 6.4% 5.6% 6.5% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1485 4533 23562 1518 4575 21374 Mean (pg/ml) 1485 4533 23562 1518 4575 21374 Standard deviation 66.82 271.98 1507.96 85 297.37 1432.05 Standard deviation 66.82 271.98 1507.96 85 297.37 1432.05 CV (%) 4.5% 6% 6.4% 5.6% 6.5% 6.7% CV (%) 4.5% 6% 6.4% 5.6% 6.5% 6.7%

Sensitivity

<50 pg/mL

Function

Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria (PubMed:7517398, PubMed:24120359) . Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide (PubMed:7517398, PubMed:24120359) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

53384 MW

Specificity

Natural and recombinant human LBP

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Lipopolysaccharide-binding protein

Subcellular Location

Secreted . Cytoplasmic granule membrane . Membrane-associated in polymorphonuclear Leukocytes (PMN) granules. .

Sequence Similarities

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein Name

Lipopolysaccharide-binding protein

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.021 0.078 0.124 0.227 0.398 0.712 1.242 1.802 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.021 0.078 0.124 0.227 0.398 0.712 1.242 1.802 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.021 0.078 0.124 0.227 0.398 0.712 1.242 1.802 O.D. 0.021 0.078 0.124 0.227 0.398 0.712 1.242 1.802

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.