Mouse HGF/Hepatocyte growth factor ELISA Kit PicoKine®

Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 248 247 243 246 2.27 0.9% Sample 2 1468 1414 1310 1392 1396 56.83 4% Sample 3 5963 5251 5451 5032 5424 344.54 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 248 247 243 246 2.27 0.9% Sample 1 249 248 247 243 246 2.27 0.9% Sample 2 1468 1414 1310 1392 1396 56.83 4% Sample 2 1468 1414 1310 1392 1396 56.83 4% Sample 3 5963 5251 5451 5032 5424 344.54 6.3% Sample 3 5963 5251 5451 5032 5424 344.54 6.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1468 5963 259 1383 5909 Standard deviation 16.18 89.54 298.15 20.72 106.49 372.26 CV (%) 6.5% 6.1% 5% 8% 7.7% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1468 5963 259 1383 5909 Standard deviation 16.18 89.54 298.15 20.72 106.49 372.26 CV (%) 6.5% 6.1% 5% 8% 7.7% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1468 5963 259 1383 5909 Mean (pg/ml) 249 1468 5963 259 1383 5909 Standard deviation 16.18 89.54 298.15 20.72 106.49 372.26 Standard deviation 16.18 89.54 298.15 20.72 106.49 372.26 CV (%) 6.5% 6.1% 5% 8% 7.7% 6.3% CV (%) 6.5% 6.1% 5% 8% 7.7% 6.3%

Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.094 0.144 0.278 0.52 0.902 1.468 2.07 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.094 0.144 0.278 0.52 0.902 1.468 2.07 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.094 0.144 0.278 0.52 0.902 1.468 2.07 O.D. 0.022 0.094 0.144 0.278 0.52 0.902 1.468 2.07

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.