Mouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine®

Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 185 189 201 196 9.53 4.8% Sample 2 665 691 673 711 685 17.72 2.5% Sample 3 1909 1945 1858 1909 1905 30.98 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 185 189 201 196 9.53 4.8% Sample 1 209 185 189 201 196 9.53 4.8% Sample 2 665 691 673 711 685 17.72 2.5% Sample 2 665 691 673 711 685 17.72 2.5% Sample 3 1909 1945 1858 1909 1905 30.98 1.6% Sample 3 1909 1945 1858 1909 1905 30.98 1.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 665 1909 199 711 1727 Standard deviation 8.56 34.58 114.54 8.55 49.77 112.25 CV (%) 4.1% 5.2% 6% 4.3% 7% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 665 1909 199 711 1727 Standard deviation 8.56 34.58 114.54 8.55 49.77 112.25 CV (%) 4.1% 5.2% 6% 4.3% 7% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 665 1909 199 711 1727 Mean (pg/ml) 209 665 1909 199 711 1727 Standard deviation 8.56 34.58 114.54 8.55 49.77 112.25 Standard deviation 8.56 34.58 114.54 8.55 49.77 112.25 CV (%) 4.1% 5.2% 6% 4.3% 7% 6.5% CV (%) 4.1% 5.2% 6% 4.3% 7% 6.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.018 0.082 0.152 0.294 0.577 1.045 1.587 2.057 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.018 0.082 0.152 0.294 0.577 1.045 1.587 2.057 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.018 0.082 0.152 0.294 0.577 1.045 1.587 2.057 O.D. 0.018 0.082 0.152 0.294 0.577 1.045 1.587 2.057

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.