Human KLK8/Kallikrein-8 ELISA Kit PicoKine®

Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 203 200 202 205 6.72 3.2% Sample 2 1843 1850 1769 1673 1783 71.38 4% Sample 3 4442 4794 4201 4701 4534 231.75 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 203 200 202 205 6.72 3.2% Sample 1 217 203 200 202 205 6.72 3.2% Sample 2 1843 1850 1769 1673 1783 71.38 4% Sample 2 1843 1850 1769 1673 1783 71.38 4% Sample 3 4442 4794 4201 4701 4534 231.75 5.1% Sample 3 4442 4794 4201 4701 4534 231.75 5.1%

Kallikrein-8; hK8;3.4.21.118; Neuropsin; NP; Ovasin; Serine protease 19; Serine protease TADG-14; Tumor-associated differentially expressed gene 14 protein; KLK8; NRPN, PRSS19, TADG14; UNQ283/PRO322

Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes. .

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1843 4442 218 1690 4088 Standard deviation 16.7 108.73 333.15 20.71 118.3 322.95 CV (%) 7.7% 7.5% 7.5% 9.5% 7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1843 4442 218 1690 4088 Standard deviation 16.7 108.73 333.15 20.71 118.3 322.95 CV (%) 7.7% 7.5% 7.5% 9.5% 7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 217 1843 4442 218 1690 4088 Mean (pg/ml) 217 1843 4442 218 1690 4088 Standard deviation 16.7 108.73 333.15 20.71 118.3 322.95 Standard deviation 16.7 108.73 333.15 20.71 118.3 322.95 CV (%) 7.7% 7.5% 7.5% 9.5% 7% 7.9% CV (%) 7.7% 7.5% 7.5% 9.5% 7% 7.9%

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.056 0.111 0.207 0.385 0.752 1.321 2.314 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.056 0.111 0.207 0.385 0.752 1.321 2.314 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.056 0.111 0.207 0.385 0.752 1.321 2.314 O.D. 0.003 0.056 0.111 0.207 0.385 0.752 1.321 2.314

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.