Human CD30/TNFRSF8 ELISA Kit PicoKine®

Human CD30/TNFRSF8 ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 321 324 313 319 319 4.02 1.2% Sample 2 1322 1420 1463 1452 1414 55.55 3.9% Sample 3 3947 3780 4218 4450 4098 256.04 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 321 324 313 319 319 4.02 1.2% Sample 1 321 324 313 319 319 4.02 1.2% Sample 2 1322 1420 1463 1452 1414 55.55 3.9% Sample 2 1322 1420 1463 1452 1414 55.55 3.9% Sample 3 3947 3780 4218 4450 4098 256.04 6.2% Sample 3 3947 3780 4218 4450 4098 256.04 6.2%

Synonyms

Tumor necrosis factor receptor superfamily member 8; CD30L receptor; Ki-1 antigen; Lymphocyte activation antigen CD30; CD30; TNFRSF8; CD30, D1S166E

Gene Name

TNFRSF8

Gene ID

943

UniProt

P28908

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F19-K379

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cell Type Markers, Cytokines, Immunology, Innate Immunity, Receptors, TNF Superfamily, Tumor Biomarkers, Tumor Immunology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 321 1322 3947 305 1371 3830 Standard deviation 25.03 64.77 157.88 28.06 93.22 214.48 CV (%) 7.8% 4.9% 4% 9.2% 6.8% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 321 1322 3947 305 1371 3830 Standard deviation 25.03 64.77 157.88 28.06 93.22 214.48 CV (%) 7.8% 4.9% 4% 9.2% 6.8% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 321 1322 3947 305 1371 3830 Mean (pg/ml) 321 1322 3947 305 1371 3830 Standard deviation 25.03 64.77 157.88 28.06 93.22 214.48 Standard deviation 25.03 64.77 157.88 28.06 93.22 214.48 CV (%) 7.8% 4.9% 4% 9.2% 6.8% 5.6% CV (%) 7.8% 4.9% 4% 9.2% 6.8% 5.6%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

63747 MW

Specificity

Natural and recombinant human CD30

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Tumor necrosis factor receptor superfamily member 8

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 6 TNFR-Cys repeats.

Protein Name

Tumor necrosis factor receptor superfamily member 8

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.086 0.12 0.202 0.33 0.552 1.035 1.955 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.086 0.12 0.202 0.33 0.552 1.035 1.955 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.036 0.086 0.12 0.202 0.33 0.552 1.035 1.955 O.D. 0.036 0.086 0.12 0.202 0.33 0.552 1.035 1.955

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.