Human Cathepsin L ELISA Kit PicoKine®

Human Cathepsin L ELISA Kit PicoKine® (96 Tests) . Quantitate Human CTSL in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin L is an important lysosomal endopeptidase enzyme which is involved in the initiation of protein degradation. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. The gene is mapped to 9q21-q22. It is a member of the Peptidase C1 family, which play an important role in diverse processes including normal lysosome mediated protein turnover, antigen and proprotein processing, and apoptosis. Cathepsin L has been reported in many organisms including fish, birds and mammals.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 202 210 182 199 10.52 5.2% Sample 2 726 748 779 754 751 18.87 2.5% Sample 3 1367 1597 1607 1555 1531 96.95 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 202 210 182 199 10.52 5.2% Sample 1 204 202 210 182 199 10.52 5.2% Sample 2 726 748 779 754 751 18.87 2.5% Sample 2 726 748 779 754 751 18.87 2.5% Sample 3 1367 1597 1607 1555 1531 96.95 6.3% Sample 3 1367 1597 1607 1555 1531 96.95 6.3%

Synonyms

Cathepsin L1;3.4.22.15; Cathepsin L; Major excreted protein; MEP; Cathepsin L1 heavy chain; Cathepsin L1 light chain; CTSL; CTSL1

Gene Name

CTSL

Gene ID

1514

UniProt

P07711

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E113-V333

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Membrane Proteins, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 726 1367 205 706 1305 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 726 1367 205 706 1305 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 204 726 1367 205 706 1305 Mean (pg/ml) 204 726 1367 205 706 1305 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 Standard deviation 10.2 29.76 66.98 11.07 36.71 82.21 CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3% CV (%) 5% 4.1% 4.9% 5.4% 5.2% 6.3%

Sensitivity

<10 pg/mL

Function

Important for the overall degradation of proteins in lysosomes.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37564 MW

Specificity

Natural and recombinant human Cathepsin L

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin L1

Subcellular Location

Lysosome.

Protein Name

Cathepsin L1

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926 O.D. 0.034 0.102 0.151 0.252 0.453 0.783 1.331 1.926

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.