Mouse IL-11/Interleukin-11 ELISA Kit PicoKine®

Interleukin 11 (IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 63 64 62 63 0.82 1.3% Sample 2 305 315 284 284 297 13.47 4.5% Sample 3 882 992 963 898 933 45.29 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 63 64 62 63 0.82 1.3% Sample 1 64 63 64 62 63 0.82 1.3% Sample 2 305 315 284 284 297 13.47 4.5% Sample 2 305 315 284 284 297 13.47 4.5% Sample 3 882 992 963 898 933 45.29 4.8% Sample 3 882 992 963 898 933 45.29 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 305 882 63 276 909 Standard deviation 4.86 16.77 62.62 4.91 16.83 70.9 CV (%) 7.6% 5.5% 7.1% 7.8% 6.1% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 305 882 63 276 909 Standard deviation 4.86 16.77 62.62 4.91 16.83 70.9 CV (%) 7.6% 5.5% 7.1% 7.8% 6.1% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 64 305 882 63 276 909 Mean (pg/ml) 64 305 882 63 276 909 Standard deviation 4.86 16.77 62.62 4.91 16.83 70.9 Standard deviation 4.86 16.77 62.62 4.91 16.83 70.9 CV (%) 7.6% 5.5% 7.1% 7.8% 6.1% 7.8% CV (%) 7.6% 5.5% 7.1% 7.8% 6.1% 7.8%

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:8913282) . Also promotes the proliferation of hepatocytes in response to liver damage (PubMed:22253262) . Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed:10026196, PubMed:23948300) . Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed:23948300, PubMed:22253262) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.105 0.173 0.268 0.467 0.761 1.27 1.966 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.105 0.173 0.268 0.467 0.761 1.27 1.966 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.014 0.105 0.173 0.268 0.467 0.761 1.27 1.966 O.D. 0.014 0.105 0.173 0.268 0.467 0.761 1.27 1.966

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.