Human VE-Cadherin CDH5 ELISA Kit PicoKine®

Cadherin 5, type 2 or VE-cadherin also known as CD144, is encoded by the gene CDH5. It is a classical cadherin from the cadherin superfamily. This gene is mapped to mouse chromosome 8. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It has found that PECAM1, VE-cadherin and VEGFR2 together are sufficient to confer responsiveness to flow in heterologous cells.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2009 2773 1726 1853 2090 85.07 4.1% Sample 2 11824 8610 11420 7806 9915 648.44 6.5% Sample 3 28737 48366 29173 43280 37389 2598.54 7.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2009 2773 1726 1853 2090 85.07 4.1% Sample 1 2009 2773 1726 1853 2090 85.07 4.1% Sample 2 11824 8610 11420 7806 9915 648.44 6.5% Sample 2 11824 8610 11420 7806 9915 648.44 6.5% Sample 3 28737 48366 29173 43280 37389 2598.54 7.0% Sample 3 28737 48366 29173 43280 37389 2598.54 7.0%

Atherosclerosis, Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Vascular Inflammation, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2009 11824 28737 1971 8782 24517 Standard deviation 77.35 308.61 1847.79 112.94 654.26 1098.01 CV (%) 3.8% 2.6% 6.4% 5.7% 7.5% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2009 11824 28737 1971 8782 24517 Standard deviation 77.35 308.61 1847.79 112.94 654.26 1098.01 CV (%) 3.8% 2.6% 6.4% 5.7% 7.5% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2009 11824 28737 1971 8782 24517 Mean (pg/ml) 2009 11824 28737 1971 8782 24517 Standard deviation 77.35 308.61 1847.79 112.94 654.26 1098.01 Standard deviation 77.35 308.61 1847.79 112.94 654.26 1098.01 CV (%) 3.8% 2.6% 6.4% 5.7% 7.5% 4.3% CV (%) 3.8% 2.6% 6.4% 5.7% 7.5% 4.3%

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.002 0.08 0.155 0.307 0.6259 1.063 1.748 2.066 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.002 0.08 0.155 0.307 0.6259 1.063 1.748 2.066 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.002 0.08 0.155 0.307 0.6259 1.063 1.748 2.066 O.D. 0.002 0.08 0.155 0.307 0.6259 1.063 1.748 2.066

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.