Human Galectin-3BP ELISA Kit PicoKine®

Human Galectin-3BP ELISA Kit PicoKine® (96 Tests) . Quantitate Human LGALS3BP in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell–cell and cell–matrix interactions. Using fluorescence in–situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 367 361 366 351 22.91 6.5% Sample 2 1090 1067 1143 1041 1085 37.57 3.4% Sample 3 4158 3836 4350 4159 4125 184.65 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 367 361 366 351 22.91 6.5% Sample 1 312 367 361 366 351 22.91 6.5% Sample 2 1090 1067 1143 1041 1085 37.57 3.4% Sample 2 1090 1067 1143 1041 1085 37.57 3.4% Sample 3 4158 3836 4350 4159 4125 184.65 4.4% Sample 3 4158 3836 4350 4159 4125 184.65 4.4%

Synonyms

Galectin-3-binding protein; Basement membrane autoantigen p105; Lectin galactoside-binding soluble 3-binding protein; Mac-2-binding protein; MAC2BP; Mac-2 BP; Tumor-associated antigen 90K; LGALS3BP; M2BP

Gene Name

LGALS3BP

Gene ID

3959

UniProt

Q08380

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V19-D585

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Monoclonal antibody from mouse

Tissue Specificity

Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. .

Applications

ELISA

Field of Research

Cancer, Cell Cycle, Cell Division, Oncoproteins, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1090 4158 326 1154 3768 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1090 4158 326 1154 3768 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1090 4158 326 1154 3768 Mean (pg/ml) 312 1090 4158 326 1154 3768 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 Standard deviation 18.72 63.22 282.74 20.86 84.24 271.29 CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2% CV (%) 6% 5.8% 6.8% 6.4% 7.3% 7.2%

Sensitivity

<10 pg/mL

Function

Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

65331 MW

Specificity

Natural and recombinant human Galectin-3BP

Anticoagulant

Heparin

Gene Name Synonym

Galectin-3-binding protein

Subcellular Location

Secreted . Secreted, extracellular space, extracellular matrix .

Protein Name

Galectin-3-binding protein

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064 O.D. 0.055 0.125 0.189 0.309 0.574 0.956 1.437 2.064

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

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