Human ADAMTS13 ELISA Kit PicoKine®

Human ADAMTS13 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ADAMTS13 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP) . ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1395 1323 1392 1567 1419 90.03 6.3% Sample 2 7236 8286 7812 8064 7849 391.89 4.9% Sample 3 20816 20291 22433 22964 21626 1104.51 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1395 1323 1392 1567 1419 90.03 6.3% Sample 1 1395 1323 1392 1567 1419 90.03 6.3% Sample 2 7236 8286 7812 8064 7849 391.89 4.9% Sample 2 7236 8286 7812 8064 7849 391.89 4.9% Sample 3 20816 20291 22433 22964 21626 1104.51 5.1% Sample 3 20816 20291 22433 22964 21626 1104.51 5.1%

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS 13; ADAM-TS13; ADAMTS-13;3.4.24.87; von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease; ADAMTS13; C9orf8; UNQ6102/PRO20085

Gene Name

ADAMTS13

Gene ID

11093

UniProt

Q76LX8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q34-W688

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Plasma. Expressed primarily in liver. .

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131322 7236 20816 1322 6617 20301 Standard deviation 104.62 289.44 1164.9 104.43 291.14 1197.75 CV (%) 7.5% 4% 5.6% 7.9% 4.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 131322 7236 20816 1322 6617 20301 Standard deviation 104.62 289.44 1164.9 104.43 291.14 1197.75 CV (%) 7.5% 4% 5.6% 7.9% 4.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 131322 7236 20816 1322 6617 20301 Mean (pg/ml) 131322 7236 20816 1322 6617 20301 Standard deviation 104.62 289.44 1164.9 104.43 291.14 1197.75 Standard deviation 104.62 289.44 1164.9 104.43 291.14 1197.75 CV (%) 7.5% 4% 5.6% 7.9% 4.4% 5.9% CV (%) 7.5% 4% 5.6% 7.9% 4.4% 5.9%

Sensitivity

<20 pg/mL

Function

Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

153604 MW

Specificity

Natural and recombinant human ADAMTS13

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

A disintegrin and metalloproteinase with thrombospondin motifs 13

Subcellular Location

Secreted . Secretion enhanced by O-fucosylation of TSP type-1 repeats.

Protein Name

A disintegrin and metalloproteinase with thrombospondin motifs 13

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.056 0.167 0.218 0.329 0.503 0.862 1.369 1.808 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.056 0.167 0.218 0.329 0.503 0.862 1.369 1.808 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.056 0.167 0.218 0.329 0.503 0.862 1.369 1.808 O.D. 0.056 0.167 0.218 0.329 0.503 0.862 1.369 1.808

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.