Human PEDF/SerpinF1 ELISA Kit PicoKine®

Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1) . In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 286 285 271 293 284 13.05 4.6% Sample 2 780 796 821 805 801 33.62 4.2% Sample 3 2612 2744 2752 2653 2690 104.92 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 286 285 271 293 284 13.05 4.6% Sample 1 286 285 271 293 284 13.05 4.6% Sample 2 780 796 821 805 801 33.62 4.2% Sample 2 780 796 821 805 801 33.62 4.2% Sample 3 2612 2744 2752 2653 2690 104.92 3.9% Sample 3 2612 2744 2752 2653 2690 104.92 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 286 780 2612 271 802 2714 Standard deviation 19.16 44.46 104 48 19.51 53.73 130.27 CV (%) 6.7% 5.7% 4% 7.2% 6.7% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 286 780 2612 271 802 2714 Standard deviation 19.16 44.46 104 48 19.51 53.73 130.27 CV (%) 6.7% 5.7% 4% 7.2% 6.7% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 286 780 2612 271 802 2714 Mean (pg/ml) 286 780 2612 271 802 2714 Standard deviation 19.16 44.46 104 48 19.51 53.73 130.27 Standard deviation 19.16 44.46 104 48 19.51 53.73 130.27 CV (%) 6.7% 5.7% 4% 7.2% 6.7% 4.8% CV (%) 6.7% 5.7% 4% 7.2% 6.7% 4.8%

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.061 0.117 0.214 0.405 0.722 1.297 1.834 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.061 0.117 0.214 0.405 0.722 1.297 1.834 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.061 0.117 0.214 0.405 0.722 1.297 1.834 O.D. 0.006 0.061 0.117 0.214 0.405 0.722 1.297 1.834

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.