Human Granzyme A ELISA Kit PicoKine®

Human Granzyme A ELISA Kit PicoKine® (96 Tests) . Quantitate Human GZMA in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface nonself antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 38 38 38 38 0.86 2.2% Sample 2 218 224 234 218 223 6.53 2.9% Sample 3 455 435 399 422 427 20.33 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 40 38 38 38 38 0.86 2.2% Sample 1 40 38 38 38 38 0.86 2.2% Sample 2 218 224 234 218 223 6.53 2.9% Sample 2 218 224 234 218 223 6.53 2.9% Sample 3 455 435 399 422 427 20.33 4.7% Sample 3 455 435 399 422 427 20.33 4.7%

Synonyms

Granzyme A;3.4.21.78; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1; Fragmentin-1; Granzyme-1; Hanukkah factor; H factor; HF; GZMA; CTLA3, HFSP

Gene Name

GZMA

Gene ID

3001

UniProt

P12544

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: I29-V262

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Apoptosis, Cell Biology, Immunology, Innate Immunity, NK Cells, T Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 218 455 42 207 410 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 40 218 455 42 207 410 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 40 218 455 42 207 410 Mean (pg/ml) 40 218 455 42 207 410 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 Standard deviation 2 13.73 27.3 2.73 17.9%5 25.42 CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2% CV (%) 5% 7.9%% 6% 6.5% 7.9% 6.2%

Sensitivity

<10 pg/mL

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

28999 MW

Specificity

Natural and recombinant human Granzyme A

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Granzyme A

Subcellular Location

Isoform alpha: Secreted. Cytoplasmic granule.

Protein Name

Granzyme A

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914 O.D. 0.058 0.188 0.282 0.409 0.716 1.056 1.533 1.914

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.