Human HO-1/HMOX1/HSP32 ELISA Kit PicoKine®

HMOX1 (heme oxygenase (decycling) 1) is a human gene that encodes for the enzyme heme oxygenase 1. It is localized to chromosome 22. Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 156 130 134 142 10.63 7.4% Sample 2 658 603 678 602 635 33.5 5.2% Sample 3 3760 3562 3498 3701 3630 104.86 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 156 130 134 142 10.63 7.4% Sample 1 149 156 130 134 142 10.63 7.4% Sample 2 658 603 678 602 635 33.5 5.2% Sample 2 658 603 678 602 635 33.5 5.2% Sample 3 3760 3562 3498 3701 3630 104.86 2.8% Sample 3 3760 3562 3498 3701 3630 104.86 2.8%

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Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 658 3760 138 610 3391 Standard deviation 11.62 32.9 180.48 12.69 39.65 200.06 CV (%) 7.8% 5% 4.8% 9.2% 6.5% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 658 3760 138 610 3391 Standard deviation 11.62 32.9 180.48 12.69 39.65 200.06 CV (%) 7.8% 5% 4.8% 9.2% 6.5% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 149 658 3760 138 610 3391 Mean (pg/ml) 149 658 3760 138 610 3391 Standard deviation 11.62 32.9 180.48 12.69 39.65 200.06 Standard deviation 11.62 32.9 180.48 12.69 39.65 200.06 CV (%) 7.8% 5% 4.8% 9.2% 6.5% 5.9% CV (%) 7.8% 5% 4.8% 9.2% 6.5% 5.9%

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.004 0.074 0.152 0.314 0.63 1.106 1.758 2.077 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.004 0.074 0.152 0.314 0.63 1.106 1.758 2.077 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.004 0.074 0.152 0.314 0.63 1.106 1.758 2.077 O.D. 0.004 0.074 0.152 0.314 0.63 1.106 1.758 2.077

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.