Mouse beta IG-H3/TGFBI ELISA Kit PicoKine®

Mouse beta IG-H3/TGFBI ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tgfbi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 301 315 329 290 308 14.66 4.7% Sample 2 1595 1475 1467 1429 1491 62.23 4.1% Sample 3 5482 6340 6191 6242 6063 340.11 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 301 315 329 290 308 14.66 4.7% Sample 1 301 315 329 290 308 14.66 4.7% Sample 2 1595 1475 1467 1429 1491 62.23 4.1% Sample 2 1595 1475 1467 1429 1491 62.23 4.1% Sample 3 5482 6340 6191 6242 6063 340.11 5.6% Sample 3 5482 6340 6191 6242 6063 340.11 5.6%

Synonyms

Transforming growth factor-beta-induced protein ig-h3; Beta ig-h3; Tgfbi

Gene Name

Tgfbi

Gene ID

21810

UniProt

P82198

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G24-H683

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in heart, kidney, liver, skeletal muscle, testis, thyroid and uterus (PubMed:8024701) . .

Applications

ELISA

Field of Research

Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Growth Factors/Hormones, Neuroscience, Organogenesis, Sensory System, Signal Transduction, Structures, Visual System

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 15302 5482 302 1576 5124 Standard deviation 21.37 95.7 241.2 22.65 100.86 312.56 CV (%) 7.1% 6% 4.4% 7.5% 6.4% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 301 15302 5482 302 1576 5124 Standard deviation 21.37 95.7 241.2 22.65 100.86 312.56 CV (%) 7.1% 6% 4.4% 7.5% 6.4% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 301 15302 5482 302 1576 5124 Mean (pg/ml) 301 15302 5482 302 1576 5124 Standard deviation 21.37 95.7 241.2 22.65 100.86 312.56 Standard deviation 21.37 95.7 241.2 22.65 100.86 312.56 CV (%) 7.1% 6% 4.4% 7.5% 6.4% 6.1% CV (%) 7.1% 6% 4.4% 7.5% 6.4% 6.1%

Sensitivity

<5 pg/mL

Function

Plays a role in cell adhesion (PubMed:8024701) . May play a role in cell-collagen interactions (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

74597 MW

Specificity

Natural and recombinant mouse beta IG-H3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Transforming growth factor-beta-induced protein ig-h3

Subcellular Location

Secreted . Secreted, extracellular space, extracellular matrix . May be associated both with microfibrils and with the cell surface. .

Protein Name

Transforming growth factor-beta-induced protein ig-h3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.074 0.144 0.267 0.556 0.966 1.492 1.992 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.074 0.144 0.267 0.556 0.966 1.492 1.992 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.074 0.144 0.267 0.556 0.966 1.492 1.992 O.D. 0.005 0.074 0.144 0.267 0.556 0.966 1.492 1.992

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.