Mouse ALCAM/CD166 ELISA Kit PicoKine®

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 132 124 119 122 6.05 4.9% Sample 2 727 733 850 715 756 54.51 7.2% Sample 3 2784 2655 2448 2326 2553 177.7 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 116 132 124 119 122 6.05 4.9% Sample 1 116 132 124 119 122 6.05 4.9% Sample 2 727 733 850 715 756 54.51 7.2% Sample 2 727 733 850 715 756 54.51 7.2% Sample 3 2784 2655 2448 2326 2553 177.7 6.9% Sample 3 2784 2655 2448 2326 2553 177.7 6.9%

Detected on brain motor neurons, in differentiating retinal ganglion cells and in adult retina (PubMed:15345243) . Detected on leukocytes and on lymphatic endothelial cells (PubMed:23169771) . Detected in spleen B cells and T-cells (at protein level) (PubMed:9209500) . Detected in adult brain and embryonic spinal cord (PubMed:15345243) . Expressed at high levels in the brain, and lung, and at lower levels in the liver, and the kidney, as well as by activated leukocytes (PubMed:9209500) . .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 727 2784 112 706 2677 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 116 727 2784 112 706 2677 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 116 727 2784 112 706 2677 Mean (pg/ml) 116 727 2784 112 706 2677 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 Standard deviation 8.58 29.8 219.93 7.4%8 32.47 211.48 CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9% CV (%) 7.4% 4.1% 7.9% 7.4% 4.6% 7.9%

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity) . Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity) . Mediates homotypic interactions with cells that express ALCAM (PubMed:24740813) . Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:23169771) . Required for normal organization of the lymph vessel network (PubMed:23169771) . Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813) . Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow (PubMed:25730656) . Promotes in vitro osteoblast proliferation and differentiation (PubMed:25730656) . Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity) . Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed:15345243) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse. .

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079 O.D. 0.028 0.099 0.147 0.261 0.462 0.834 1.387 2.079

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.