Mouse FGF23 ELISA Kit PicoKine®

Mouse FGF23 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Fgf23 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 29 31 34 32 2.38 7.4% Sample 2 216 235 249 229 232 11.86 5.1% Sample 3 449 395 424 400 417 21.48 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 29 31 34 32 2.38 7.4% Sample 1 35 29 31 34 32 2.38 7.4% Sample 2 216 235 249 229 232 11.86 5.1% Sample 2 216 235 249 229 232 11.86 5.1% Sample 3 449 395 424 400 417 21.48 5.1% Sample 3 449 395 424 400 417 21.48 5.1%

Synonyms

Fibroblast growth factor 23; FGF-23; Fgf23

Gene Name

Fgf23

Gene ID

64654

UniProt

Q9EPC2

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Y25-V251

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Mainly expressed in the brain and thymus at low levels. In brain; preferentially expressed in the ventrolateral thalamic nucleus.

Applications

ELISA

Field of Research

Cancer, Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Lineage Markers, Lineage Specification, Signal Transduction, Stem Cells, Structures

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 449 33 213 449 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 CV (%) 7.8% 4.1% 7.9% 8.4% 6% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 449 33 213 449 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 CV (%) 7.8% 4.1% 7.9% 8.4% 6% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 216 449 33 213 449 Mean (pg/ml) 35 216 449 33 213 449 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 Standard deviation 2.73 8.85 35.47 2.77 12.78 35.47 CV (%) 7.8% 4.1% 7.9% 8.4% 6% 7.9% CV (%) 7.8% 4.1% 7.9% 8.4% 6% 7.9%

Sensitivity

<10 pg/mL

Function

Regulator of phosphate homeostasis (By similarity) . Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity) . Acts ly on the parathyroid to decrease PTH secretion (By similarity) . Regulator of vitamin-D metabolism (By similarity) . Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity) . Upregulates EGR1 expression in the presence of KL. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

27758 MW

Specificity

Natural and recombinant mouse FGF23

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fibroblast growth factor 23

Subcellular Location

Secreted . Secretion is dependent on O-glycosylation. .

Protein Name

Fibroblast growth factor 23

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.076 0.163 0.336 0.619 1.057 1.672 2.306 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.076 0.163 0.336 0.619 1.057 1.672 2.306 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.076 0.163 0.336 0.619 1.057 1.672 2.306 O.D. 0.004 0.076 0.163 0.336 0.619 1.057 1.672 2.306

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.