Mouse CXCL9/Mig ELISA Kit PicoKine®

Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family that is also known as Monokine induced by gamma interferon (MIG) . CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. CXCL9, CXCL10 and CXCL11 all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. The standard product used in this kit is recombinant human CXCL9, consisting of 103 amino acids with the molecular mass of 11.7KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 84 92 82 86 3.74 4.3% Sample 2 228 207 217 212 216 7.77 3.5% Sample 3 891 891 830 879 872 25.16 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 86 84 92 82 86 3.74 4.3% Sample 1 86 84 92 82 86 3.74 4.3% Sample 2 228 207 217 212 216 7.77 3.5% Sample 2 228 207 217 212 216 7.77 3.5% Sample 3 891 891 830 879 872 25.16 2.8% Sample 3 891 891 830 879 872 25.16 2.8%

C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Monokine induced by interferon-gamma; MIG; MuMIG; Protein m119; Small-inducible cytokine B9; Cxcl9; Mig, Scyb9

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 297 1865 96 268 1558 Standard deviation 3.89 9.92 98.66 4.86 9.11 36.77 CV (%) 4.1% 3.3% 5.3% 5.1% 3.4% 2.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 297 1865 96 268 1558 Standard deviation 3.89 9.92 98.66 4.86 9.11 36.77 CV (%) 4.1% 3.3% 5.3% 5.1% 3.4% 2.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 297 1865 96 268 1558 Mean (pg/ml) 95 297 1865 96 268 1558 Standard deviation 3.89 9.92 98.66 4.86 9.11 36.77 Standard deviation 3.89 9.92 98.66 4.86 9.11 36.77 CV (%) 4.1% 3.3% 5.3% 5.1% 3.4% 2.4% CV (%) 4.1% 3.3% 5.3% 5.1% 3.4% 2.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.011 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.011 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.011 O.D. 0.009 0.06 0.107 0.195 0.357 0.681 1.255 2.011

Aliquot 0.1 ml per well of the 4,000 pg/ml, 2,000 pg/ml, 1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml mouse CXCL9 standard solutions into the precoated 96-well plate. Add 0.1 ml of the sample diluent buffer into the control well (Zero well) . Add 0.1 ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma (EDTA) to each empty well. See “Sample Dilution Guideline” above for details. It is recommended that each mouse CXCL9 standard solution and each sample be measured in duplicate.

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.