Human DCR3/TNFRSF6B ELISA Kit PicoKine®

Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A. Overexpression of this gene has been noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 246 272 280 267 12.83 4.8% Sample 2 1644 1634 1754 1881 1728 99.97 5.7% Sample 3 5011 5645 5121 5650 5356 293.34 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 246 272 280 267 12.83 4.8% Sample 1 272 246 272 280 267 12.83 4.8% Sample 2 1644 1634 1754 1881 1728 99.97 5.7% Sample 2 1644 1634 1754 1881 1728 99.97 5.7% Sample 3 5011 5645 5121 5650 5356 293.34 5.4% Sample 3 5011 5645 5121 5650 5356 293.34 5.4%

Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1644 5011 272 1715 5192 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1644 5011 272 1715 5192 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1644 5011 272 1715 5192 Mean (pg/ml) 272 1644 5011 272 1715 5192 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 Standard deviation 12.24 1441.324 330.72 14.96 145.77 441.32 CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5% CV (%) 4.5% 7.6% 6.6% 5.5% 8.5% 8.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411 O.D. 0.047 0.11 0.174 0.312 0.577 1.014 1.808 2.411

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.