Human ADAM8/Ms2 ELISA Kit PicoKine®

A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 150 153 145 147 3.96 2.6% Sample 2 493 533 465 527 504 27.43 5.4% Sample 3 1805 1971 1907 1936 1904 61.88 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 143 150 153 145 147 3.96 2.6% Sample 1 143 150 153 145 147 3.96 2.6% Sample 2 493 533 465 527 504 27.43 5.4% Sample 2 493 533 465 527 504 27.43 5.4% Sample 3 1805 1971 1907 1936 1904 61.88 3.2% Sample 3 1805 1971 1907 1936 1904 61.88 3.2%

Apoptosis, Cancer, Cell Biology, Cell Death, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Receptors, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 493 1805 150 511 1879 Standard deviation 10.72 97.47 97.47 11.25 33.72 127.77 CV (%) 7.5% 5.6% 5.4% 7.5% 6.6% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 143 493 1805 150 511 1879 Standard deviation 10.72 97.47 97.47 11.25 33.72 127.77 CV (%) 7.5% 5.6% 5.4% 7.5% 6.6% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 143 493 1805 150 511 1879 Mean (pg/ml) 143 493 1805 150 511 1879 Standard deviation 10.72 97.47 97.47 11.25 33.72 127.77 Standard deviation 10.72 97.47 97.47 11.25 33.72 127.77 CV (%) 7.5% 5.6% 5.4% 7.5% 6.6% 6.8% CV (%) 7.5% 5.6% 5.4% 7.5% 6.6% 6.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.095 0.194 0.302 0.603 0.99 1.456 2.002 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.095 0.194 0.302 0.603 0.99 1.456 2.002 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.016 0.095 0.194 0.302 0.603 0.99 1.456 2.002 O.D. 0.016 0.095 0.194 0.302 0.603 0.99 1.456 2.002

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.