Human NRCAM ELISA Kit PicoKine®

Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes ional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during ional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 111 114 128 118 6.68 5.6% Sample 2 814 761 742 769 771 26.42 3.4% Sample 3 1867 2040 1721 2087 1928 145.25 7.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 122 111 114 128 118 6.68 5.6% Sample 1 122 111 114 128 118 6.68 5.6% Sample 2 814 761 742 769 771 26.42 3.4% Sample 2 814 761 742 769 771 26.42 3.4% Sample 3 1867 2040 1721 2087 1928 145.25 7.5% Sample 3 1867 2040 1721 2087 1928 145.25 7.5%

Neuronal cell adhesion molecule; Nr-CAM; Neuronal surface protein Bravo ; hBravo ; NgCAM-related cell adhesion molecule; Ng-CAM-related; NRCAM; KIAA0343

Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 814 1867 115 889 1724 Standard deviation 5.49 35 102.68 5.75 54.22 125.85 CV (%) 4.5% 4.3% 5.5% 5% 6.1% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 122 814 1867 115 889 1724 Standard deviation 5.49 35 102.68 5.75 54.22 125.85 CV (%) 4.5% 4.3% 5.5% 5% 6.1% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 122 814 1867 115 889 1724 Mean (pg/ml) 122 814 1867 115 889 1724 Standard deviation 5.49 35 102.68 5.75 54.22 125.85 Standard deviation 5.49 35 102.68 5.75 54.22 125.85 CV (%) 4.5% 4.3% 5.5% 5% 6.1% 7.3% CV (%) 4.5% 4.3% 5.5% 5% 6.1% 7.3%

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.083 0.148 0.277 0.515 0.87 1.686 2.529 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.083 0.148 0.277 0.515 0.87 1.686 2.529 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.014 0.083 0.148 0.277 0.515 0.87 1.686 2.529 O.D. 0.014 0.083 0.148 0.277 0.515 0.87 1.686 2.529

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.