Human Relaxin-3/RLN3 ELISA Kit PicoKine®

Relaxin-3 is a neuropeptide which is highly conserved in species ranging from flies, fish, rodents and humans. It is a member and ancestral gene of the relaxin family of peptides, which includes the namesake hormone relaxin (designated 'H2 relaxin' in humans) which mediates peripheral actions during pregnancy and which was found to relax the pelvic ligament in guinea pigs almost a century ago. The broad distribution of relaxin-3 fibres/RXFP3 within several key neuronal circuits suggests an ability to modulate a broad range of behaviours. This has been confirmed in a growing number of rodent studies, which demonstrate relaxin-3 is able to modulate arousal, the response to stress, feeding/metabolism and memory; and likely plays a role in the generation/regulation of hippocampal theta rhythm.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 66 66 70 66 1.92 2.9% Sample 2 225 219 209 235 222 9.43 4.2% Sample 3 942 1017 854 1030 960 70.19 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 66 66 70 66 1.92 2.9% Sample 1 65 66 66 70 66 1.92 2.9% Sample 2 225 219 209 235 222 9.43 4.2% Sample 2 225 219 209 235 222 9.43 4.2% Sample 3 942 1017 854 1030 960 70.19 7.3% Sample 3 942 1017 854 1030 960 70.19 7.3%

Relaxin-3; Insulin-like peptide INSL7; Insulin-like peptide 7; Prorelaxin H3; Relaxin-3 B chain; Relaxin-3 A chain; RLN3; INSL7, RXN3, ZINS4; UNQ6188/PRO20213

Cancer, Developmental Biology, Endocrine System, Growth Factors/Hormones, Metabolism, Neurology Process, Neuropeptides, Neuroscience, Neurotransmitter, Reproduction, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 225 942 60 227 930 Standard deviation 4.42 15.07 63.11 7.7% 18.38 71.61 CV (%) 6.8% 6.7% 6.7% 8.5% 8.1% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 225 942 60 227 930 Standard deviation 4.42 15.07 63.11 7.7% 18.38 71.61 CV (%) 6.8% 6.7% 6.7% 8.5% 8.1% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 65 225 942 60 227 930 Mean (pg/ml) 65 225 942 60 227 930 Standard deviation 4.42 15.07 63.11 7.7% 18.38 71.61 Standard deviation 4.42 15.07 63.11 7.7% 18.38 71.61 CV (%) 6.8% 6.7% 6.7% 8.5% 8.1% 7.7% CV (%) 6.8% 6.7% 6.7% 8.5% 8.1% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.084 0.163 0.225 0.34 0.534 0.84 1.407 2.315 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.084 0.163 0.225 0.34 0.534 0.84 1.407 2.315 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.084 0.163 0.225 0.34 0.534 0.84 1.407 2.315 O.D. 0.084 0.163 0.225 0.34 0.534 0.84 1.407 2.315

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.