Mouse Cripto/TDGF1 ELISA Kit PicoKine®

Mouse Cripto/TDGF1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Tdgf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 110 114 115 112 2.27 2% Sample 2 661 633 642 685 655 19.92 3% Sample 3 1213 1047 1166 1231 1164 71.73 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 110 114 115 112 2.27 2% Sample 1 110 110 114 115 112 2.27 2% Sample 2 661 633 642 685 655 19.92 3% Sample 2 661 633 642 685 655 19.92 3% Sample 3 1213 1047 1166 1231 1164 71.73 6.1% Sample 3 1213 1047 1166 1231 1164 71.73 6.1%

Synonyms

Teratocarcinoma-derived growth factor; Cripto growth factor; Epidermal growth factor-like Cripto protein; Tdgf1; Cripto

Gene Name

Tdgf1

Gene ID

21667

UniProt

P51865

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: R26-Q150

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.

Applications

ELISA

Field of Research

Embryonic Stem Cells, Lineage Markers, Lineage Specification, Secreted, Stem Cells

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 661 1213 107 721 1176 Mean (pg/ml) 110 661 1213 107 721 1176 Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% Standard deviation 7.5%7 27.1 73.99 4.7%4 37.49 84.9% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5% CV (%) 4.7% 4.1% 6.1% 4.9% 5.2% 7.5%

Sensitivity

<10 pg/mL

Function

Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

18754 MW

Specificity

Natural and recombinant mouse Cripto

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Teratocarcinoma-derived growth factor

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor .

Protein Name

Teratocarcinoma-derived growth factor

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.