Human Stanniocalcin 1 / STC1 / STC ELISA Kit PicoKine®

Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 70 70 78 73 3.41 4.6% Sample 2 279 279 267 314 284 17.58 6.1% Sample 3 1061 980 888 961 972 61.56 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 70 70 78 73 3.41 4.6% Sample 1 75 70 70 78 73 3.41 4.6% Sample 2 279 279 267 314 284 17.58 6.1% Sample 2 279 279 267 314 284 17.58 6.1% Sample 3 1061 980 888 961 972 61.56 6.3% Sample 3 1061 980 888 961 972 61.56 6.3%

Expressed in most tissues, with the highest levels in ovary, prostate, heart, kidney and thyroid. In the kidney, expression is confined to the nephron, specifically in the distal convoluted tubule and in the collecting tubule. Not detected in the brain, liver, spleen, peripheral blood leukocytes and adrenal medulla.

Cancer, Cell Biology, Cofactors, Vitamins/Minerals, Growth Factors/Hormones, Metabolism, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction, Vitamins/Minerals

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 279 1061 71 306 1123 Standard deviation 3.9 13.11 56.23 4.04 19.27 60.64 CV (%) 5.2% 4.7% 5.3% 5.7% 6.3% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 75 279 1061 71 306 1123 Standard deviation 3.9 13.11 56.23 4.04 19.27 60.64 CV (%) 5.2% 4.7% 5.3% 5.7% 6.3% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 75 279 1061 71 306 1123 Mean (pg/ml) 75 279 1061 71 306 1123 Standard deviation 3.9 13.11 56.23 4.04 19.27 60.64 Standard deviation 3.9 13.11 56.23 4.04 19.27 60.64 CV (%) 5.2% 4.7% 5.3% 5.7% 6.3% 5.4% CV (%) 5.2% 4.7% 5.3% 5.7% 6.3% 5.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.099 0.145 0.205 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.099 0.145 0.205 0.348 0.6254 1.135 1.946 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.056 0.099 0.145 0.205 0.348 0.6254 1.135 1.946 O.D. 0.056 0.099 0.145 0.205 0.348 0.6254 1.135 1.946

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.