Mouse Complement Factor H/CFH ELISA Kit PicoKine®

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation') . This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1657 1662 1609 1498 1606 65.97 4.1% Sample 2 6025 6672 6459 5668 6206 388.38 6.2% Sample 3 19536 22857 19293 22922 21152 1739.77 8.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1657 1662 1609 1498 1606 65.97 4.1% Sample 1 1657 1662 1609 1498 1606 65.97 4.1% Sample 2 6025 6672 6459 5668 6206 388.38 6.2% Sample 2 6025 6672 6459 5668 6206 388.38 6.2% Sample 3 19536 22857 19293 22922 21152 1739.77 8.2% Sample 3 19536 22857 19293 22922 21152 1739.77 8.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1657 6025 19536 1601 5467 19303 Standard deviation 120.96 343.42 1133.08 124.87 371.75 1351.21 CV (%) 7.3% 5.7% 5.8% 7.8% 6.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1657 6025 19536 1601 5467 19303 Standard deviation 120.96 343.42 1133.08 124.87 371.75 1351.21 CV (%) 7.3% 5.7% 5.8% 7.8% 6.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1657 6025 19536 1601 5467 19303 Mean (pg/ml) 1657 6025 19536 1601 5467 19303 Standard deviation 120.96 343.42 1133.08 124.87 371.75 1351.21 Standard deviation 120.96 343.42 1133.08 124.87 371.75 1351.21 CV (%) 7.3% 5.7% 5.8% 7.8% 6.8% 7% CV (%) 7.3% 5.7% 5.8% 7.8% 6.8% 7%

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b) NBB complex (C5 convertase) in the alternative complement pathway. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.035 0.121 0.22 0.391 0.671 1.043 1.608 2.051 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.035 0.121 0.22 0.391 0.671 1.043 1.608 2.051 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.035 0.121 0.22 0.391 0.671 1.043 1.608 2.051 O.D. 0.035 0.121 0.22 0.391 0.671 1.043 1.608 2.051

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.