Human ERBB3/Her3 ELISA Kit PicoKine®

Receptor tyrosine-protein kinase erbB-3, also known as HER3 (human epidermal growth factor receptor 3), is a membrane bound protein that in humans is encoded by the ERBB3 gene. ErbB3 has been shown to bind the ligands heregulin and NRG-2. Ligand binding causes a change in conformation that allows for dimerization, phosphorylation, and activation of signal transduction. ErbB3 can heterodimerize with any of the other three ErbB family members. The theoretical ErbB3 homodimer would be non-functional because the kinase-impaired protein requires transphosporylation by its binding partner to be active. Unlike the other ErbB receptor tyrosine kinase family members which are activated through autophosphorylation upon ligand binding, ErbB3 is found to be kinase impaired, having only 1/1000th the autophosphorylation activity of EGFR and no ability to phosphorylate other proteins. Therefore, ErbB3 must act as anallosteric activator.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 249 242 239 244 4.38 1.7% Sample 2 1903 1635 1831 1793 1790 98.08 5.4% Sample 3 5498 5793 5614 5244 5537 199.27 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 249 249 242 239 244 4.38 1.7% Sample 1 249 249 242 239 244 4.38 1.7% Sample 2 1903 1635 1831 1793 1790 98.08 5.4% Sample 2 1903 1635 1831 1793 1790 98.08 5.4% Sample 3 5498 5793 5614 5244 5537 199.27 3.5% Sample 3 5498 5793 5614 5244 5537 199.27 3.5%

Apoptosis, Cell Biology, Developmental Biology, Growth Factors/Hormones, Intracellular, Kinases, Nervous System Development, Organogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1903 5498 238 1734 5885 Standard deviation 12.45 125.59 324.38 16.42 114.44 406.06 CV (%) 5% 6.6% 5.9% 6.9% 6.6% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 249 1903 5498 238 1734 5885 Standard deviation 12.45 125.59 324.38 16.42 114.44 406.06 CV (%) 5% 6.6% 5.9% 6.9% 6.6% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 249 1903 5498 238 1734 5885 Mean (pg/ml) 249 1903 5498 238 1734 5885 Standard deviation 12.45 125.59 324.38 16.42 114.44 406.06 Standard deviation 12.45 125.59 324.38 16.42 114.44 406.06 CV (%) 5% 6.6% 5.9% 6.9% 6.6% 6.9% CV (%) 5% 6.6% 5.9% 6.9% 6.6% 6.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.159 0.208 0.313 0.525 0.905 1.519 2.28 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.159 0.208 0.313 0.525 0.905 1.519 2.28 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.072 0.159 0.208 0.313 0.525 0.905 1.519 2.28 O.D. 0.072 0.159 0.208 0.313 0.525 0.905 1.519 2.28

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.