Mouse Epiregulin / EREG ELISA Kit PicoKine®

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 196 173 198 192 189 9.9 5.2% Sample 2 1010 1076 1107 932 1031 67.16 6.5% Sample 3 4381 3736 4082 3649 3962 291.12 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 196 173 198 192 189 9.9 5.2% Sample 1 196 173 198 192 189 9.9 5.2% Sample 2 1010 1076 1107 932 1031 67.16 6.5% Sample 2 1010 1076 1107 932 1031 67.16 6.5% Sample 3 4381 3736 4082 3649 3962 291.12 7.3% Sample 3 4381 3736 4082 3649 3962 291.12 7.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 196 1010 4381 179 1069 4367 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 196 1010 4381 179 1069 4367 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 196 1010 4381 179 1069 4367 Mean (pg/ml) 196 1010 4381 179 1069 4367 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 Standard deviation 11.76 66.5%5 2213.961 13.96 73.76 231.45 CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3% CV (%) 6% 6.5% 5.2% 7.8% 6.9% 5.3%

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (By similarity) . Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813 O.D. 0.005 0.019 0.047 0.085 0.178 0.41 0.879 1.813

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.