Human Epiregulin ELISA Kit PicoKine®

Epiregulin (EPR) is a protein that in humans is encoded by the EREG gene. It is a member of the epidermal growth factor family. Epiregulin can function as a ligand of epidermal growth factor receptor (EGFR), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. The secondary structure at the C-terminus epiregulin is different from other epidermal growth factor family ligands because of the lack of hydrogen bonds. The structural difference at the C-terminus may provide an explanation for the reduced binding affinity of epiregulin to the ERBB receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 13 14 12 12 12 0.82 6.8% Sample 2 57 55 60 52 56 2.91 5.1% Sample 3 113 120 129 118 120 5.78 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 13 14 12 12 12 0.82 6.8% Sample 1 13 14 12 12 12 0.82 6.8% Sample 2 57 55 60 52 56 2.91 5.1% Sample 2 57 55 60 52 56 2.91 5.1% Sample 3 113 120 129 118 120 5.78 4.8% Sample 3 113 120 129 118 120 5.78 4.8%

In normal adults, expressed predominantly in the placenta and peripheral blood leukocytes. High levels were detected in carcinomas of the bladder, lung, kidney and colon. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 13 57 113 12 56 105 Standard deviation 0.78 4.21 6.19 0.94 4.48 6.4 CV (%) 6% 7.4% 4.6% 7.9% 8% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 13 57 113 12 56 105 Standard deviation 0.78 4.21 6.19 0.94 4.48 6.4 CV (%) 6% 7.4% 4.6% 7.9% 8% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 13 57 113 12 56 105 Mean (pg/ml) 13 57 113 12 56 105 Standard deviation 0.78 4.21 6.19 0.94 4.48 6.4 Standard deviation 0.78 4.21 6.19 0.94 4.48 6.4 CV (%) 6% 7.4% 4.6% 7.9% 8% 6.1% CV (%) 6% 7.4% 4.6% 7.9% 8% 6.1%

Ligand of the EGF receptor/EGFR and ERBB4. Stimulates EGFR and ERBB4 tyrosine phosphorylation (PubMed:9419975) . Contributes to inflammation, wound healing, tissue repair, and oocyte maturation by regulating angiogenesis and vascular remodeling and by stimulating cell proliferation (PubMed:24631357) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.014 0.073 0.138 0.277 0.501 0.87 1.686 2.529 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.014 0.073 0.138 0.277 0.501 0.87 1.686 2.529 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.014 0.073 0.138 0.277 0.501 0.87 1.686 2.529 O.D. 0.014 0.073 0.138 0.277 0.501 0.87 1.686 2.529

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.