Human LOXL2/Lysyl Oxidase Like 2 ELISA Kit PicoKine®

Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 291 276 299 279 17.89 6.4% Sample 2 1465 1420 1599 1469 1488 66.77 4.4% Sample 3 3854 3928 3993 3849 3906 59.17 1.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 252 291 276 299 279 17.89 6.4% Sample 1 252 291 276 299 279 17.89 6.4% Sample 2 1465 1420 1599 1469 1488 66.77 4.4% Sample 2 1465 1420 1599 1469 1488 66.77 4.4% Sample 3 3854 3928 3993 3849 3906 59.17 1.5% Sample 3 3854 3928 3993 3849 3906 59.17 1.5%

Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. Up- regulated in a number of cancers cells and tissues.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1465 3854 263 1490 4228 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 CV (%) 4.7% 7.8% 6.6% 5.3% 8.3% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 252 1465 3854 263 1490 4228 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 CV (%) 4.7% 7.8% 6.6% 5.3% 8.3% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 252 1465 3854 263 1490 4228 Mean (pg/ml) 252 1465 3854 263 1490 4228 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 Standard deviation 11.84 114.27 254.36 13.93 123.67 317.1 CV (%) 4.7% 7.8% 6.6% 5.3% 8.3% 7.5% CV (%) 4.7% 7.8% 6.6% 5.3% 8.3% 7.5%

Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) . When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted, extracellular space, extracellular matrix, basement membrane . Nucleus. Chromosome. Associated with chromatin. It is unclear how LOXL2 is nuclear: it contains a clear signal sequence and is predicted to localize in the extracellular medium. However, different reports confirmed the intracellular location and its key role in transcription regulation.

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417 O.D. 0.005 0.034 0.059 0.119 0.277 0.68 1.642 2.417

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.