Human TGFBR3/Tgf Beta Riii ELISA Kit PicoKine®

Human TGFBR3/Tgf Beta Riii ELISA Kit PicoKine® (96 Tests) . Quantitate Human TGFBR3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell- surfacechondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. Betaglycan binds to various members of the TGF-beta superfamily of ligands via its core protein, and bFGF via its heparan sulfate chains. It is not involved ly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. By cDNA array and immunohistochemistry analyses, it is found that TGFBR3 expression is lost in most breast cancers examined in association with loss of heterozygosity of the TGFBR3 locus. TGFBR3 expression decreased during breast cancer progression, and low levels predicted decreased recurrence-free survival in patients. Loss of TGFBR3 through allelic imbalance is a frequent genetic event during breast cancer development that increases metastatic potential. Also, TGFBR3 can localize transformation in the heart and play an essential, nonredundant role in TGF-beta signaling.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 199 223 196 201 13.58 6.7% Sample 2 1370 1157 1296 1222 1261 79.75 6.3% Sample 3 5554 6224 5744 5545 5766 275.69 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 186 199 223 196 201 13.58 6.7% Sample 1 186 199 223 196 201 13.58 6.7% Sample 2 1370 1157 1296 1222 1261 79.75 6.3% Sample 2 1370 1157 1296 1222 1261 79.75 6.3% Sample 3 5554 6224 5744 5545 5766 275.69 4.7% Sample 3 5554 6224 5744 5545 5766 275.69 4.7%

Synonyms

Transforming growth factor beta receptor type 3; TGF-beta receptor type 3; TGFR-3; Betaglycan; Transforming growth factor beta receptor III; TGF-beta receptor type III; TGFBR3

Gene Name

TGFBR3

Gene ID

7049

UniProt

Q03167

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G21-D781

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Signaling Pathways, Stem Cells, Surface Molecules, TGF Beta

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1370 5554 182 1499 5882 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 186 1370 5554 182 1499 5882 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 186 1370 5554 182 1499 5882 Mean (pg/ml) 186 1370 5554 182 1499 5882 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 Standard deviation 8.74 102.75 294.36 11.83 133.41 376.44 CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4% CV (%) 4.7% 7.5% 5.3% 6.5% 8.9% 6.4%

Sensitivity

<10 pg/mL

Function

Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

93499 MW

Specificity

Natural and recombinant human TGFBR3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Transforming growth factor beta receptor type 3

Subcellular Location

Secreted. Secreted, extracellular space. Cell membrane ; Single-pass type I membrane protein . Exists both as a membrane-bound form and as soluble form in serum and in the extracellular matrix.

Protein Name

Transforming growth factor beta receptor type 3

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098 O.D. 0.063 0.095 0.133 0.216 0.358 0.72 1.405 2.098

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.