Mouse FGF21 ELISA Kit PicoKine®

Mouse FGF21 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Fgf21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 62 61 54 60 3.76 6.2% Sample 2 320 285 310 296 302 13.32 4.4% Sample 3 918 928 964 981 947 25.71 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 64 62 61 54 60 3.76 6.2% Sample 1 64 62 61 54 60 3.76 6.2% Sample 2 320 285 310 296 302 13.32 4.4% Sample 2 320 285 310 296 302 13.32 4.4% Sample 3 918 928 964 981 947 25.71 2.7% Sample 3 918 928 964 981 947 25.71 2.7%

Synonyms

Fibroblast growth factor 21; FGF-21; Fgf21

Gene Name

Fgf21

Gene ID

56636

UniProt

Q9JJN1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A29-S210

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Most abundantly expressed in the liver, also expressed in the thymus at lower levels. .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 320 918 65 289 938 Standard deviation 2.81 24 52.32 3.12 27.16 70.35 CV (%) 4.4% 7.5% 5.7% 4.8% 9.4% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 64 320 918 65 289 938 Standard deviation 2.81 24 52.32 3.12 27.16 70.35 CV (%) 4.4% 7.5% 5.7% 4.8% 9.4% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 64 320 918 65 289 938 Mean (pg/ml) 64 320 918 65 289 938 Standard deviation 2.81 24 52.32 3.12 27.16 70.35 Standard deviation 2.81 24 52.32 3.12 27.16 70.35 CV (%) 4.4% 7.5% 5.7% 4.8% 9.4% 7.5% CV (%) 4.4% 7.5% 5.7% 4.8% 9.4% 7.5%

Sensitivity

<10 pg/mL

Function

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) . Activity probably requires the presence of KLB. .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23237 MW

Specificity

Natural and recombinant mouse FGF21

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fibroblast growth factor 21

Subcellular Location

Secreted .

Protein Name

Fibroblast growth factor 21

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.031 0.055 0.116 0.238 0.439 0.937 1.88 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.031 0.055 0.116 0.238 0.439 0.937 1.88 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.009 0.031 0.055 0.116 0.238 0.439 0.937 1.88 O.D. 0.009 0.031 0.055 0.116 0.238 0.439 0.937 1.88

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.