Human Relaxin 1 ELISA Kit PicoKine®

Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 144 125 144 133 136 8.01 5.8% Sample 2 819 759 734 688 750 47.28 6.3% Sample 3 2390 2222 2307 2378 2324 67.01 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 144 125 144 133 136 8.01 5.8% Sample 1 144 125 144 133 136 8.01 5.8% Sample 2 819 759 734 688 750 47.28 6.3% Sample 2 819 759 734 688 750 47.28 6.3% Sample 3 2390 2222 2307 2378 2324 67.01 2.8% Sample 3 2390 2222 2307 2378 2324 67.01 2.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 144 819 2390 133 796 2573 Standard deviation 10.94 37.67 100.38 10.5 42.98 118.35 CV (%) 7.6% 4.6% 4.2% 7.9% 5.4% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 144 819 2390 133 796 2573 Standard deviation 10.94 37.67 100.38 10.5 42.98 118.35 CV (%) 7.6% 4.6% 4.2% 7.9% 5.4% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 144 819 2390 133 796 2573 Mean (pg/ml) 144 819 2390 133 796 2573 Standard deviation 10.94 37.67 100.38 10.5 42.98 118.35 Standard deviation 10.94 37.67 100.38 10.5 42.98 118.35 CV (%) 7.6% 4.6% 4.2% 7.9% 5.4% 4.6% CV (%) 7.6% 4.6% 4.2% 7.9% 5.4% 4.6%

Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals. May be involved in remodeling of connective tissues during pregnancy, promoting growth of pubic ligaments and ripening of the cervix.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.088 0.132 0.207 0.401 0.881 1.648 2.295 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.088 0.132 0.207 0.401 0.881 1.648 2.295 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.025 0.088 0.132 0.207 0.401 0.881 1.648 2.295 O.D. 0.025 0.088 0.132 0.207 0.401 0.881 1.648 2.295

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.