Human Tenascin-C/TNC ELISA Kit PicoKine®

Tenascin C (TN-C) is a glycoprotein that in humans is encoded by the TNC gene. It is expressed in the extracellular matrix of various tissues during development, disease or injury, and in restricted neurogenic areas of the central nervous system. Tenascin-C is the founding member of the gene family (Tenascin) . In the embryo it is made by migrating cells like the neural crest; it is also abundant in developing tendons, bone and cartilage. TN-C clearly plays a role in cell signaling as evidenced by its ability to be induced during events such as trauma, inflammation, or cancer development. Also, TN-C is important in regulating cell proliferation and migration, especially during developmental differentiation and wound healing.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 114 94 112 103 9.52 9.2% Sample 2 868 820 876 826 847 24.75 2.9% Sample 3 3019 2632 2824 3047 2880 167.21 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 94 114 94 112 103 9.52 9.2% Sample 1 94 114 94 112 103 9.52 9.2% Sample 2 868 820 876 826 847 24.75 2.9% Sample 2 868 820 876 826 847 24.75 2.9% Sample 3 3019 2632 2824 3047 2880 167.21 5.8% Sample 3 3019 2632 2824 3047 2880 167.21 5.8%

Tenascin; TN; Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; JI; Myotendinous antigen; Neuronectin; Tenascin-C; TN-C; TNC; HXB

Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Neural Signal Transduction, Neural Stem Cells, Neurology Process, Neuroscience, Secreted Molecules, Signal Transduction, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 868 3019 89 892 3055 Standard deviation 4.51 61.62 223.4 5.51 74.03 226.07 CV (%) 4.8% 7.1% 7.4% 6.2% 8.3% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 868 3019 89 892 3055 Standard deviation 4.51 61.62 223.4 5.51 74.03 226.07 CV (%) 4.8% 7.1% 7.4% 6.2% 8.3% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 868 3019 89 892 3055 Mean (pg/ml) 94 868 3019 89 892 3055 Standard deviation 4.51 61.62 223.4 5.51 74.03 226.07 Standard deviation 4.51 61.62 223.4 5.51 74.03 226.07 CV (%) 4.8% 7.1% 7.4% 6.2% 8.3% 7.4% CV (%) 4.8% 7.1% 7.4% 6.2% 8.3% 7.4%

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.001 0.023 0.052 0.109 0.235 0.512 1.033 1.887 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.001 0.023 0.052 0.109 0.235 0.512 1.033 1.887 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.001 0.023 0.052 0.109 0.235 0.512 1.033 1.887 O.D. 0.001 0.023 0.052 0.109 0.235 0.512 1.033 1.887

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.