Human KLK12/Klk L5 ELISA Kit PicoKine®

Human KLK12/Klk L5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human KLK12 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 208 206 217 216 211 4.81 2.2% Sample 2 1283 1297 1371 1367 1329 39.83 2.9% Sample 3 4339 4587 4086 4442 4363 182.83 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 208 206 217 216 211 4.81 2.2% Sample 1 208 206 217 216 211 4.81 2.2% Sample 2 1283 1297 1371 1367 1329 39.83 2.9% Sample 2 1283 1297 1371 1367 1329 39.83 2.9% Sample 3 4339 4587 4086 4442 4363 182.83 4.1% Sample 3 4339 4587 4086 4442 4363 182.83 4.1%

Synonyms

Kallikrein-12;3.4.21.-; Kallikrein-like protein 5; KLK-L5; KLK12; KLKL5; UNQ669/PRO1303

Gene Name

KLK12

Gene ID

43849

UniProt

Q9UKR0

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-N248

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 208 1283 4339 215 1293 3970 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 208 1283 4339 215 1293 3970 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 208 1283 4339 215 1293 3970 Mean (pg/ml) 208 1283 4339 215 1293 3970 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 Standard deviation 8.94 92.37 260.34 9.67 93.09 273.93 CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9% CV (%) 4.3% 7.2% 6% 4.5% 7.2% 6.9%

Sensitivity

<10 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

26734 MW

Specificity

Natural and recombinant human KLK12

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Kallikrein-12

Subcellular Location

Secreted.

Protein Name

Kallikrein-12

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024 O.D. 0.019 0.095 0.191 0.389 0.697 1.274 1.745 2.024

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.