Human KLK11/Kallikrein-11 ELISA Kit PicoKine®

Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 206 233 215 217 9.73 4.4% Sample 2 1054 1031 1100 1045 1057 25.86 2.4% Sample 3 4898 4850 4518 5291 4889 274.24 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 217 206 233 215 217 9.73 4.4% Sample 1 217 206 233 215 217 9.73 4.4% Sample 2 1054 1031 1100 1045 1057 25.86 2.4% Sample 2 1054 1031 1100 1045 1057 25.86 2.4% Sample 3 4898 4850 4518 5291 4889 274.24 5.6% Sample 3 4898 4850 4518 5291 4889 274.24 5.6%

Kallikrein-11; hK11;3.4.21.-; Hippostasin; Serine protease 20; Trypsin-like protease; Kallikrein-11 inactive chain 1; Kallikrein-11 inactive chain 2; KLK11; PRSS20, TLSP; UNQ649/PRO1279

Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level) . .

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1054 4888 232 994 4808 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 CV (%) 4.9% 7.9% 4.9% 7.3% 9.3% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1054 4888 232 994 4808 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 CV (%) 4.9% 7.9% 4.9% 7.3% 9.3% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 217 1054 4888 232 994 4808 Mean (pg/ml) 217 1054 4888 232 994 4808 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 CV (%) 4.9% 7.9% 4.9% 7.3% 9.3% 5.4% CV (%) 4.9% 7.9% 4.9% 7.3% 9.3% 5.4%

Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.073 0.115 0.206 0.386 0.678 1.116 1.898 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.073 0.115 0.206 0.386 0.678 1.116 1.898 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.032 0.073 0.115 0.206 0.386 0.678 1.116 1.898 O.D. 0.032 0.073 0.115 0.206 0.386 0.678 1.116 1.898

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.