Human IL-17RA ELISA Kit PicoKine®

Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. The IL17R gene is mapped to 22q11.22-q11.23 by radiation hybrid analysis. Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Also, it is showed that mouse Il17r bound HSV-13 and mouse Il17. Recombinant soluble Il17r inhibited mitogen-induced T-cell proliferation and Il2 production.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 78 90 82 83 4.33 5.2% Sample 2 276 303 258 288 281 16.48 5.8% Sample 3 1012 1007 960 871 962 56.58 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 84 78 90 82 83 4.33 5.2% Sample 1 84 78 90 82 83 4.33 5.2% Sample 2 276 303 258 288 281 16.48 5.8% Sample 2 276 303 258 288 281 16.48 5.8% Sample 3 1012 1007 960 871 962 56.58 5.8% Sample 3 1012 1007 960 871 962 56.58 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 276 1012 83 250 1067 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 84 276 1012 83 250 1067 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 84 276 1012 83 250 1067 Mean (pg/ml) 84 276 1012 83 250 1067 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 Standard deviation 6.63 19.32 72.86 7.3 19.25 87.49 CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2% CV (%) 7.9% 7% 7.2% 8.8% 7.7% 8.2%

Receptor for IL17A (PubMed:17911633, PubMed:9367539) . Receptor for IL17F (PubMed:19838198, PubMed:17911633) . Binds to IL17A with higher affinity than to IL17F (PubMed:17911633) . Binds IL17A and IL17F homodimers as part of a heterodimeric complex with IL17RC (PubMed:16785495) . Also binds heterodimers formed by IL17A and IL17F as part of a heterodimeric complex with IL17RC (PubMed:18684971) . Receptor for IL17C as part of a heterodimeric complex with IL17RE (PubMed:21993848) . Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6 (PubMed:16785495, PubMed:17911633, PubMed:18684971) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982 O.D. 0.019 0.078 0.129 0.222 0.4 0.671 1.198 1.982

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.