Human DAN/NBL1 ELISA Kit PicoKine®

Human DAN/NBL1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human NBL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 158 176 186 157 169 12.27 7.2% Sample 2 931 1035 907 1065 984 66.89 6.7% Sample 3 5529 5905 6039 5194 5666 330.83 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 158 176 186 157 169 12.27 7.2% Sample 1 158 176 186 157 169 12.27 7.2% Sample 2 931 1035 907 1065 984 66.89 6.7% Sample 2 931 1035 907 1065 984 66.89 6.7% Sample 3 5529 5905 6039 5194 5666 330.83 5.8% Sample 3 5529 5905 6039 5194 5666 330.83 5.8%

Synonyms

Neuroblastoma suppressor of tumorigenicity 1; DAN domain family member 1; Protein N03; Zinc finger protein DAN; NBL1; DAN, DAND1

Gene Name

NBL1

Gene ID

4681

UniProt

P41271

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-D181

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Most abundant in normal lung and meningioma.

Applications

ELISA

Field of Research

Cancer, Growth Factors/Hormones, Oncoproteins/Suppressors, Signal Transduction, Tumor Suppressors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 158 931 5529 160 963 5394 Standard deviation 9.63 70.75 293.03 10.08 75.6%1 302.06 CV (%) 6.1% 7.6% 5.3% 7.8%% 7.8% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 158 931 5529 160 963 5394 Standard deviation 9.63 70.75 293.03 10.08 75.6%1 302.06 CV (%) 6.1% 7.6% 5.3% 7.8%% 7.8% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 158 931 5529 160 963 5394 Mean (pg/ml) 158 931 5529 160 963 5394 Standard deviation 9.63 70.75 293.03 10.08 75.6%1 302.06 Standard deviation 9.63 70.75 293.03 10.08 75.6%1 302.06 CV (%) 6.1% 7.6% 5.3% 7.8%% 7.8% 5.6% CV (%) 6.1% 7.6% 5.3% 7.8%% 7.8% 5.6%

Sensitivity

<10 pg/mL

Function

Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

19408 MW

Specificity

Natural and recombinant human DAN

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Neuroblastoma suppressor of tumorigenicity 1

Subcellular Location

Secreted.

Protein Name

Neuroblastoma suppressor of tumorigenicity 1

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.105 0.167 0.295 0.575 1.022 1.783 2.234 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.105 0.167 0.295 0.575 1.022 1.783 2.234 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.008 0.105 0.167 0.295 0.575 1.022 1.783 2.234 O.D. 0.008 0.105 0.167 0.295 0.575 1.022 1.783 2.234

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.