Mouse CCL21/6Ckine/Ccl21a ELISA Kit PicoKine®

Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC) . By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 46 49 42 2.23 5.3% Sample 2 159 142 224 189 179 4.14 2.3% Sample 3 678 595 676 741 673 21.25 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 37 46 49 42 2.23 5.3% Sample 1 36 37 46 49 42 2.23 5.3% Sample 2 159 142 224 189 179 4.14 2.3% Sample 2 159 142 224 189 179 4.14 2.3% Sample 3 678 595 676 741 673 21.25 3.2% Sample 3 678 595 676 741 673 21.25 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 159 678 61 128 620 Standard deviation 1.49 3.43 18.65 4.40 7.08 34.10 CV (%) 4.1% 2.2% 2.8% 7.2% 5.5% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 159 678 61 128 620 Standard deviation 1.49 3.43 18.65 4.40 7.08 34.10 CV (%) 4.1% 2.2% 2.8% 7.2% 5.5% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 159 678 61 128 620 Mean (pg/ml) 36 159 678 61 128 620 Standard deviation 1.49 3.43 18.65 4.40 7.08 34.10 Standard deviation 1.49 3.43 18.65 4.40 7.08 34.10 CV (%) 4.1% 2.2% 2.8% 7.2% 5.5% 5.5% CV (%) 4.1% 2.2% 2.8% 7.2% 5.5% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.068 0.109 0.191 0.384 0.835 1.735 3.228 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.068 0.109 0.191 0.384 0.835 1.735 3.228 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.03 0.068 0.109 0.191 0.384 0.835 1.735 3.228 O.D. 0.03 0.068 0.109 0.191 0.384 0.835 1.735 3.228

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.