Rat MIP-2 ELISA Kit PicoKine®

Rat MIP-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Cxcl2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 35 36 35 0.43 1.2% Sample 2 171 176 185 176 177 5.04 2.8% Sample 3 537 483 467 489 494 26.09 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 35 35 36 35 0.43 1.2% Sample 1 35 35 35 36 35 0.43 1.2% Sample 2 171 176 185 176 177 5.04 2.8% Sample 2 171 176 185 176 177 5.04 2.8% Sample 3 537 483 467 489 494 26.09 5.2% Sample 3 537 483 467 489 494 26.09 5.2%

Synonyms

C-X-C motif chemokine 2; Cytokine-induced neutrophil chemoattractant 3; CINC-3; Macrophage inflammatory protein 2; MIP2; Cxcl2; Cinc3, Mip-2, Mip2, Scyb2

Gene Name

Cxcl2

Gene ID

114105

UniProt

P30348

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S32-N100

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

At least expressed in the lung and trachea.

Applications

ELISA

Field of Research

Chemokines, Immunology, Innate Immunity

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 171 537 31 178 563 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 171 537 31 178 563 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 171 537 31 178 563 Mean (pg/ml) 35 171 537 31 178 563 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 Standard deviation 2.69 2.89 21.48 2.88 8.36 27.02 CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8% CV (%) 7.7% 4.6% 4% 9.3% 4.7% 4.8%

Sensitivity

<10 pg/mL

Function

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10783 MW

Specificity

Natural and recombinant rat MIP-2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-X-C motif chemokine 2

Subcellular Location

Secreted.

Protein Name

C-X-C motif chemokine 2

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294 O.D. 0.047 0.112 0.159 0.276 0.509 0.905 1.549 2.294

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.