Mouse CD86/B7-2 ELISA Kit PicoKine®

Cluster of Differentiation 86 (also known as CD86 and B7-2) is aprotein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86, like CD80, was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 175 168 168 172 4.71 2.7% Sample 2 772 787 740 767 766 16.97 2.2% Sample 3 1639 1511 1669 1837 1664 116.17 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 179 175 168 168 172 4.71 2.7% Sample 1 179 175 168 168 172 4.71 2.7% Sample 2 772 787 740 767 766 16.97 2.2% Sample 2 772 787 740 767 766 16.97 2.2% Sample 3 1639 1511 1669 1837 1664 116.17 6.9% Sample 3 1639 1511 1669 1837 1664 116.17 6.9%

Adaptive Immunity, Cell Type Markers, Hematopoietic Progenitors, Host-Virus Interaction, Immunology, Interspecies Interaction, Lymphoid, Microbiology, Myeloid, Stem Cells, T Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 772 1639 165 834 1677 Standard deviation 11.09 412.546 86.86 12.54 62.55 95.58 CV (%) 6.2% 6.2% 5.3% 7.6% 7.5% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 179 772 1639 165 834 1677 Standard deviation 11.09 412.546 86.86 12.54 62.55 95.58 CV (%) 6.2% 6.2% 5.3% 7.6% 7.5% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 179 772 1639 165 834 1677 Mean (pg/ml) 179 772 1639 165 834 1677 Standard deviation 11.09 412.546 86.86 12.54 62.55 95.58 Standard deviation 11.09 412.546 86.86 12.54 62.55 95.58 CV (%) 6.2% 6.2% 5.3% 7.6% 7.5% 5.7% CV (%) 6.2% 6.2% 5.3% 7.6% 7.5% 5.7%

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.013 0.093 0.177 0.327 0.681 1.091 1.82 2.441 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.013 0.093 0.177 0.327 0.681 1.091 1.82 2.441 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.013 0.093 0.177 0.327 0.681 1.091 1.82 2.441 O.D. 0.013 0.093 0.177 0.327 0.681 1.091 1.82 2.441

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.