Mouse TNFsR I ELISA Kit PicoKine®

Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha) . The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 39 42 39 38 2.48 6.5% Sample 2 224 227 240 237 232 6.67 2.8% Sample 3 495 438 426 477 459 28.06 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 39 42 39 38 2.48 6.5% Sample 1 35 39 42 39 38 2.48 6.5% Sample 2 224 227 240 237 232 6.67 2.8% Sample 2 224 227 240 237 232 6.67 2.8% Sample 3 495 438 426 477 459 28.06 6.1% Sample 3 495 438 426 477 459 28.06 6.1%

Tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor 1; TNF-R1; Tumor necrosis factor receptor type I; TNF-RI; TNFR-I; p55; p60; CD120a; Tnfrsf1a; Tnfr-1, Tnfr1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 224 437 37 204 459 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 224 437 37 204 459 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 224 437 37 204 459 Mean (pg/ml) 35 224 437 37 204 459 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 Standard deviation 1.5 13.88 27.22 1.7 18%2 27.08 CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9% CV (%) 4.3% 6.2% 5.5% 4.6% 8% 5.9%

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297 O.D. 0.025 0.121 0.188 0.34 0.629 1.036 1.742 2.297

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.